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Occlusal trauma is defined as an injury resulting in tissue changes within the attachment apparatus as a result of occlusal forces. It has been proved in many studies that occlusal trauma can cause a variety of destructive biological effects on periodontal tissues (1-4). It has been suggested that occlusal trauma causes various destructive effects on the periodontal tissue and two theories have been proposed relating to occlusal trauma: one is the “co-destructive factor theory” by Glickman (5), which suggests inflammatory changes induced by infection with periodontal pathogens and occlusal trauma caused by excessive occlusal loading both greatly contribute to the progression of periodontal diseases, especially those characterized by a large amount of alveolar bone resorption; and the other is the theory proposed by Waerhaug (6), which denies occlusal trauma as a co-factor in the loss of connective tissue attachment and vertical alveolar bone resorption. More recently, studies on animal models have examined research concerning occlusal trauma using monkeys (12). The experiment combined different levels of inflammation and different types of trauma. Specifically, the authors ligated the molars of monkeys using a cotton thread to induce severe inflammation, and then the monkeys were divided into two groups: one received traumatic force and the other nothing. The resulting histopathological reports indicated the more severe the periodontal tissue inflammation, the greater the tissue destruction that resulted from the traumatic occlusion, and that more severe tissue destruction occurred when traumatic force was applied in two directions, rather than one (12). Experimental mechanical stress causes changes in periodontal tissues. This has been reported by previous study (13) from an orthodontic point of view. According to the results, the changes in the pressured side of periodontal tissues were severe. However, if the cytotoxic stress is in short term, the mechanism for repairing the periodontal ligaments could be observed (13). There are still many unknowns because experimental validations have been performed mostly from a histopathological standpoint, and experimental validation of the cellular kinetics of periodontal tissues in an in vivo experimental system has not made progress (7-12). These research reports failed to performed sufficient validation on the cytological kinetics of the periodontal tissues in the case of occlusal trauma. In the view of establishing an animal experimental system that is highly versatile and repeatable, we built an experimental system in which overload is added to the molar region of mice, and we reexamined the periodontal tissues from the viewpoint of cytological kinetics (23). We then performed histopathological and also immunohistochemical examinations.. Biofield therapies are very popular in biomedical heath care systems. We conducted a case-control study of 401 patients with schizophrenia and 606 healthy subjects. The connections between tested SNPs and clinical variables (PANSS buy Neurontin age of onset, a family history, and suicide attempts) were also examined.. Twenty-four hours after drug treatment, cells were harvested and adjusted to a confluency of 0.5×106 to 1×106 cells/mL. Subsequently, cells were fixed with 70% pre-colded alcohol and stained with propidium iodide (PI). PI-labeled cells were analyzed using flow cytometry.

Twenty-four hours after drug treatment, cells were harvested and adjusted to a confluency of 0.5×106 to 1×106 cells/mL. Subsequently, cells were fixed with 70% pre-colded alcohol and stained with propidium iodide (PI). PI-labeled cells were analyzed using flow cytometry..

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